Nrf2在有氧运动训练中对小鼠骨骼肌抗氧化能力的作用     

何诗依  张缨 《北京体育大学学报》2017,40(12):50-54

摘要：目的：Nrf2是机体氧化还原平衡的主要调节因子，本研究试图探讨Nrf2在有氧运动训练中对小鼠骨骼肌抗氧化能力的作用。方法：8周龄野生C57BL/6J小鼠和Nrf2敲除小鼠40只，雌雄各半。进一步各为安静组和运动组，每组10只，共4组：分别为野生安静组（WC），野生运动组（WE），敲除安静组（KC）和敲除运动组（KE）。运动组进行4周运动强度为70%VO2max跑速的跑台运动，每周运动6 d，每天运动1 h。运动组最后一次运动后休息48 h脱颈处死，取小鼠后腿骨骼肌。RT-PCR测量Nrf2及Nrf2介导的抗氧化基因mRNA表达量；Western blot法测定骨骼肌Nrf2及抗氧化蛋白含量； GSH试剂盒测量GSH/GSSG比值。结果：1）在安静情况下，KC组和WC组相比，骨骼肌中GSR、NQO1、GCLc、GPX、HO-1和SOD2这6个抗氧化蛋白表达以及GSH/GSSG值没有显著性改变。 2）4周有氧运动训练之后，Nrf2敲除运动组的大部分抗氧化蛋白（CAT, NQO1-1, GCLc, HO-1 and SOD2）和GSH/GSSG值都要显著低于野生运动组。结论：4周70%VO2max强度的有氧运动训练，增加了成年Nrf2敲除和野生鼠骨骼肌抗氧化蛋白表达量和GSH/GSSG比值的差异，而在无训练安静情况下在两种鼠中这些指标的差异不大。说明Nrf2对有氧运动训练中骨骼肌的抗氧化反应起着非常重要的作用。  相似文献   

竞技游泳运动员运动性伤病情况调查分析          下载免费PDF全文

檀志宗  楼俊华  李男 《体育科研》2019,(6):93-97

采用问卷调查法对浙沪两省市194名游泳运动员进行运动性伤病调查，主要从运动性伤病的类型、部位、性质方面对比分析不同竞技水平游泳运动员的伤病表型特征。结果显示：优秀运动员运动性损伤较常见，多以慢性劳损为主，损伤部位依次为肩关节、腰背部和膝关节；一般运动员常发生急性损伤，损伤部位依次为膝关节、肩关节和腰背部。两组运动员鼻炎和中耳炎均常见。因此，依据运动性伤病的可能原因与机制，需要制定有针对性的预防措施，以减少运动性伤病的发生。  相似文献   

生活方式干预对心血管疾病危险因素的影响——美国HELPS项目成果     

Peter H. Brubaker  罗曦娟  Chelsea Boltj  Shuchang Du 《北京体育大学学报》2017,40(6):48-53

摘要：心血管疾病（CVD）一直是全世界最主要的死因。由于CVD等慢性疾病对健康的威胁越来越大，有必要开展生活方式干预项目以控制这些疾病的危险因素。本研究的目的是评估美国的重要慢病干预项目—HELPS治疗型生活方式改变（TLC）项目对减少CVD危险因素和其他慢性疾病的有效性。方法：140位大学教职工参加为期12周的HELPS TLC项目，干预前后用配对t检验对其体重、BMI、腰围（WC）、6分钟步行距离（6MWD）、空腹血脂和血糖进行测定和比较。结果：干预后6MWD显著增加，总胆固醇（TC）和WC显著下降。对“高危”参与者进一步分析显示：BMI（-1.5±0.5，-3.9%）、TC（- 22.8±4.0，-9.0%）、LDL（- 14.1±4.9，9.3%）和血糖（-6.9±2.2，-6.1%）均显著下降；男性腰围（-4.5±0.8，-10%）显著减小，女性未见显著改变（-1.5±0.5，-4.0%）。此外，体重的变化与TC（r=0.43）、LDL（r=0.35）和血糖（r=0.44）的变化均显著相关。结论：参加12周的生活方式干预项目可以使CVD危险因素减少，特别是在CVD“高危”个体中。HELPS TLC项目的远期效果还待进一步深入研究。  相似文献   

Compensatory changes in physical activity and sedentary time in children and adolescents with cystic fibrosis     

Kelly A. Mackintosh  Nicola D. Ridgers  Melitta A. McNarry 《Journal of sports sciences》2019,37(13):1506-1511

Physical activity (PA) is a key element in Cystic Fibrosis (CF) treatment strategies, yet little is known as to whether activity compensation occurs. This study examined whether PA and/or sedentary time on one day were temporally associated with time spent in these intensities the following day in youth with CF. Time spent sedentary and in different PA intensities were objectively-measured for seven consecutive days in 50 youth (22 boys; 12.0 ± 2.7 years); 25 with mild-to-moderate CF and 25 age- and sex-matched controls. Multilevel analyses (day and child) were conducted using generalised linear latent and mixed models. On any given day, every additional 10 minutes spent in sedentary time or moderate-to-vigorous physical activity (MVPA) were associated with 1.9 (95%CI: ?3.6 to ?1.2) and 12.4 (95%CI: ?22.1 to ?2.9) minutes less sedentary time the following day, respectively. These temporal associations were also observed when split by group (3.1 vs. 1.9 minutes for healthy and CF, respectively). These findings indicate that youth do not compensate their PA, irrespective of disease status, between days, but may compensate their sedentary time between days. Experimental studies are warranted to fully elucidate whether compensatory responses to PA and sedentary time occur, which is fundamental for informing PA promotion strategies.  相似文献   

月亮女神及其语言、神话、文化失落——中国神话原生态研究之三     

申江 《昭通师范高等专科学校学报》2002,(4)

文化人类学方法可以确认《九歌·少司命》主角的月亮女神身份。“娥奔月”系由语言疾病导致的假神话 ,其背景则是女神在神话、文化中的地位失落  相似文献   

面向决策的“城市病”诊治情报服务探索     

李纲  李阳 《图书情报工作》2016,60(14):121-127

[目的/意义]探究面向决策的“城市病”诊治情报服务模式有利于对“城市病”问题进行综合把脉,帮助城市管理者迅速找准病因、症结,从而提升社会治理效率。[方法/过程]在总结“城市病”诊治相关理论与实践现状的基础上,从宏观环境（信息环境）、中观资源（大数据战略）与用户需求（决策需求）3个层面归纳“城市病”诊治情报服务的推动力,探讨“城市病”诊治情报服务体系的组织架构以及运行机制,指出“城市病”诊治情报服务应是环环相扣、动态演进、递进循环的工程化流程。[结果/结论]情报服务的介入可以促使“城市病”诊治过程更为精细化、系统化与协同化,为政府城市管理与决策提供极为有益的信息决策参考。按照工程化情报服务思维,“城市病”诊治思路将从被动的“应对”向主动的“服务”转变。  相似文献   

Reliability of arterial spin labelling measurements of perfusion within the quadriceps during steady-state exercise     

Jonathan Fulford  Anni Vanhatalo 《European Journal of Sport Science》2016,16(1):80-87

Arterial spin labelling (ASL) provides a potential method to non-invasively determine muscle blood flow and examine the impact of interventions such as supplementation and training. However, it's a method with intrinsically low signal, leading to limitations in accuracy and temporal resolution. To examine these limitations, the current study measured perfusion via ASL on three occasions in the rectus femoris of 10 healthy adults, during light and moderate exercise, over three different exercise durations. For data sampled over 9 min, light intensity exercise gave an average perfusion of 35.0 ± 5.1 ml/min.100g^?1 with a coefficient of variation (COV) of 16% and single intraclass correlation coefficient (ICC) of 0.67. For the moderate bout, perfusion was 51.3 ± 5.6 ml/min.100g^?1 (COV 10%, ICC 0.82). When the same data were analyzed over 5 min 24 s, perfusion was 37.8 ± 11.13 (COV 30%, ICC 0.13) during light and 49.5 ± 8.8 ml/min.100g^?1 (COV 18%, ICC 0.52) during moderate exercise. When sampling was reduced to 1 min 48 s, perfusion was 41.2 ± 13.7 (COV 33%, ICC 0.26) during light and 49.5 ± 13.6 ml/min.100g^?1 (COV 28%, ICC 0.04) during moderate exercise. For 9 min a significant perfusion difference was found between the exercise intensities; however, this was not the case for sampling over 5 min 24 s or 1 min 48 s. Such findings illustrate the potential of ASL to non-invasively monitor muscle perfusion under steady-state conditions, but highlight that extended exercise protocols are necessary in order to generate date of sufficient reliability to be able to discriminate intervention dependent perfusion differences.  相似文献   

Low-intensity electrical muscle stimulation induces significant increases in muscle strength and cardiorespiratory fitness     

Toshiaki Miyamoto  Hiroyuki Kamada  Akira Tamaki  Toshio Moritani 《European Journal of Sport Science》2016,16(8):1104-1110

The aim of this study was to investigate the effect of low-intensity exercise training using belt electrode skeletal muscle electrical stimulation on muscle strength and cardiorespiratory fitness in healthy subjects. Nineteen healthy subjects were allocated into control or intervention groups; in both groups the participants kept regular physical activity while the intervention group underwent 30 min B-SES training at 3–4 METs for four weeks. Knee extensor muscle strength and cardiorespiratory endurance during incremental exercise test were measured at baseline and after four weeks for all participants. The relative change of knee extensor muscle strength in the intervention group was significantly higher than control group (p?p?相似文献   

Effects of load mass carried in a backpack upon respiratory muscle fatigue     

Mark Faghy  Sam Blacker  Peter I. Brown 《European Journal of Sport Science》2016,16(8):1032-1038

Purpose: The purpose of this study was to investigate whether loads carried in a backpack, with a load mass ranging from 0 to 20?kg, causes respiratory muscle fatigue. Methods: Eight males performed four randomised load carriage (LC) trials comprising 60?min walking at 6.5?km?h^?1 wearing a backpack of either 0 (LC₀), 10 (LC₁₀), 15 (LC₁₅) or 20?kg (LC₂₀). Inspiratory (P_Imax) and expiratory (P_Emax) mouth pressures were assessed prior to and immediately following each trial. Pulmonary gas exchange, heart rate (HR), blood lactate and glucose concentration and perceptual responses were recorded during the first and final 60?s of each trial. Results: Group mean P_Imax and P_Emax were unchanged following 60-min load carriage in all conditions (p?>?.05). There was an increase over time in pulmonary gas exchange, HR and perceptions of effort relative to baseline measures during each trial (p?p?>?.05). Conclusions: These findings indicate that sub-maximal walking with no load or carrying 10, 15 or 20?kg in a backpack for up to 60?min does not cause respiratory muscle fatigue despite causing an increase in physiological, metabolic and perceptual parameters.  相似文献   

我校566名学生健康调查分析     

詹秋霞  陈红玲  孙雪春  黄少玉 《宁德师专学报(自然科学版)》1997,(1)

本文通过调查发现我校学生中患病率以视力不良为最高,其次是龋齿、砂眼,应引起重视。  相似文献